Journal: Cell Reports

Article Title: Microglial identity and inflammatory responses are controlled by the combined effects of neurons and astrocytes

doi: 10.1016/j.celrep.2021.108882

Figure Lengend Snippet:

Article Snippet: 3 days after microglial seeding, the media was replaced with pHrodoTM Red E. Coli BioParticlesTM conjugate suspension (1:10 dilution in PBS) prepared following manufacturer’s instructions (ThermoFisher) and then incubated in a CO 2 -free incubator at 37°C for 1 h. The cells were then physically dissociated from the culture dishes, stained with Alexa Flour conjugated 647 anti-rat CD11b antibody (Biolegend) and analyzed on a BD AccuriTM C6 flow cytometer (BD biosciences).

Techniques: Plasmid Preparation, Recombinant, Isolation, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software